Épigénomique

L'épigénomique est l'étude des modifications de la régulation et de l'expression des gènes, indépendantes de leurs séquences. IntegraGen propose une gamme complète de services d'analyses de méthylation par différentes méthodes : sur puce ou par séquençage haut-débit (RRBS, MethySeq, MeDIP, ChiP-Seq).

Profil de méthylation sur puce (humain)

La puce HumanMethylation 450 d’Illumina est souvent l’outil le plus rentable pour l’information fournie, pour la plupart des études de profil de méthylation chez l’homme. Cette puce interroge plus de 450 000 marqueurs CpG sur l’ensemble du génome.

Avec des centaines d’échantillons traités chaque année depuis 2008, et en tant que prestataire privilégié de la Ligue Nationale contre le Cancer dans le cadre du programme Carte d’Identité des Tumeurs, IntegraGen est le prestataire de choix pour vos études de méthylation.

CE QUI FAIT D’INTEGRAGEN VOTRE MEILLEUR PARTENAIRE

Expertise dans la conception de projet de recherche en méthylation, sur puce ou par séquençage haut-débit
Support dans la prise en main et le traitement biostatistique des données
Traitement bisulfite inclus pour chacune des méthodes proposées

GARANTIES TECHNIQUES

Contrôles qualité qualitatifs et quantitatifs pour tous les échantillons ADN
Illumina Certified Service Provider

RRBS – Reduced Representation Bisulfite Sequencing

Le RRBS est une technique idéale pour analyser le profil de méthylation d’un échantillon par séquençage hau-débit. La technique est basée sur l’enrichissement des régions riches en CG du génome, permettant ainsi de réduite significativement la quantité de séquences et le coût associé, tout en permettant une analyse exhaustive de la majorité des promoteurs et des régions génomiques d’intérêt.

NOTRE EXPERIENCE

Préparation de banques optimisées, basées sur le protocole de Meissner et al.
Publications de nos clients disponibles sur des résultats d’études RRBS

CE QUI FAIT DE NOUS VOTRE MEILLEUR PARTENAIRE

Expertise dans le design de projets de recherche et mise en place de plan d’analyse
Protocoles RRBS optimisés pour l’homme et la souris
Digestion Msp1 ou double digestion (eRRBS)
Expertise en RRoxBS

Nous proposons également les services d’experts pour vous accompagner dans l’analyse biostatistique et l’interprétation de vos données de méthylation via notre service de pointe GeCo (Advanced Genomics Consulting).

GARANTIES TECHNIQUES

Taux de conversion >99,5%
Profondeur de séquences et couverture garanties, projet dépendant.

Bioinformatique et analyses des données

Le rendu standard inclut les données brutes et les tables annotées de résultats (taux de méthylation par marqueur CpG)
Analyse bioinformatique primaire des données avec le pipeline de référence BS-Seeker
Le rendu standard inclut le taux de méthylation par cytosine dans les 3 contextes de séquences CG/CHG/CHH
Analyse statistique avancée des données disponible avec notre service GeCo (Advanced Genomic Consulting)

Contactez-nous

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